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The production model of the Gallardo Spyder was presented at the Los Angeles Auto Show in January 2006. It is considered by the company to be a completely new model with 520 hp (513 kW hp/382) and a lower proportion of six-speed manual transmission. The soft top is fully retractable.

Aerosmith drummer Joey Kramer was the first in North America to accept delivery of the Gallardo Spyder.

Top Gear presenter Jeremy Clarkson announced on 18 February 2007, he had bought a Gallardo Spyder, replacing his Ford GT.